The aim of this study was to develop novel mucoadhesive system by the one-pot preparation method. Previous article in issue. For coating the liposomes with a pectin layer, the purified pectin was dissolved at a concentration of 0. Antimicrobial activity tests were used to determine the activity of the fractions obtained from Sep-Pak SPE and the inhibitory activity of the resulting peptide.
This can be explained by the fact that although the WPI is negatively charged, it does not completely neutralize the positive charge of the liposome on which the pectin may be adsorbed subsequently, as reflected by the increase in the size and a change in the zeta potential close to that of the pectin.
For the purpose of GI tract targeting, liposomes can be manipulated by the inclusion of polymer coatings on the liposomal surface.
Disease localization dictates the need for maximal intestinal tissue drug exposure while systemic delivery should be minimized to avoid unwanted side effects.
These results confirmed the effectiveness of pectin coating. Metronidazole exhibits its antimicrobial activity through reduction of its nitro group and the formation of toxic derivatives, inducing the death of susceptible microorganisms through interacting with DNA.
During the current study, 80 mg of MccJ25 were utilized produced from two batches. At pH 7, no significant change in the liposome size was observed, indicating inefficient coating due to repulsions between the negative charges of whey and liposomes. The cationic liposomes showed a significantly lower degree of encapsulation efficiency after coating compared with anionic liposomes.
Results and Discussion Production of the Microcin The production of MccJ25 was monitored by determining the protein concentration and bactericidal activity.
Inoculated agar was poured into sterile Petri plates and allowed to solidify for 10—15 min in a biological hood. Oral administration of immunoliposomes has its challenges, as the antibodies are prone to degradation by the stomach acid as well as by GI tract enzymes.
Liposomes have been studied for over 40 years as drug delivery systems for various routes of drug administration, including the vaginal route [ 2 ].
The fraction that showed activity was frozen and then lyophilized. Protein adsorption onto alginate-pectin microparticles and films produced by ionic gelation. Thus, we can conclude that the double coating provided protection to the microcin throughout the gastrointestinal tract.
The samples were diluted with filtered 0. However, very limited studies have been performed on improving the effectiveness of liposomes encapsulation by coating materials.
A production technique for MccJ25 has been developed, including rapid two-step purification, that allowed the application of this molecule in different fields food, medical, and veterinary where the need for such new molecules is increasingly urgent Sable et al.
Therefore, the use of nanotechnology in formulation design has been investigated as a way to further improve the efficacy of therapeutics by allowing specific targeting and uptake into inflamed tissue within the colon Jani et al.
Coating the Cationic Liposomes The data on the size and the zeta potential of the cationic coated liposomes are presented in Figure 3. Oral formulations can be designed to achieve either a local or systemic delivery of therapeutics. The complexes formed between mucin and the HM-pectin-coated liposomes were the largest, while the smallest were formed with the LM-pectin-coated liposomes.
These studies are usually limited to in vitro or ex vivo cell association experiments, and have shown positive results in specificity of cell targeting and uptake. The phosphorus standard solution was used to prepare a standard curve.
The infrared spectra of the KBr tablets were recorded using a Nicolet spectrometer Madison, WI equipped with a deuterated triglycine sulfate detector DTGS continuously purged with dry air.
The original vesicle size was reduced by sonication. The images showed that pectin helped to maintain the shape and integrity of the liposomes Figure 6c. Then, the dissolution was performed in simulated intestinal fluid SIF: Thus, the WPI and pectin were proved to be two very interesting materials for improving the encapsulation and release of antimicrobial peptides.
The dispersions were diluted with 1 mM NaCl, which was previously filtered through nm Minisart filters, to achieve a count rate between and kcps [ 16 ]. When the anionic liposomes were coated with WPI followed by pectin, the form of vesicles was much more elongated as compared to the case of coating with pectin followed by WPI where the liposomes preserved their original shape.
Natural Polymers—Chitosan and Pectin Chitosan and pectin are natural polymers that are considered non-toxic, biodegradable, and mucoadhesive. In particular, pectin has been reported as one of the main raw materials to obtain edible films by its natural abundance, low cost and renewable character.
The latest innovations in food packaging by the use of pectin-based edible films are reviewed in this paper, with special focus on the use of pectin as base material for edible coatings. FIGURE 6 | TEM of anionic liposomes, (a) non-coated, (b) WPI-coated liposomes, (c) pectin-coated liposomes, (d) pectin/WPI coated liposomes, and (e) WPI/ pectin coated liposomes.
Preparation, characterization and evaluation of bufalin liposomes coated with citrus pectin Liposomal drugs are agents which are first approved and widely used in the pharmaceutical field since it has many advantages including the affinity to biological membrane and the ability to Coating of the liposomes with pectin resulted in a.
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methoxylated pectin (HM-pectin), alginate, chitosan and hydrophobically modified ethyl The aim of this study was to identify/develop a formulation that would combine advantages of prolonged mucoadhesion with high water sorption capacity.
Thirteen different formulations Coating the liposomes with polymers Commercially available. Studies on pectin-coated liposomes and their interaction with mucin. Author links open overlay panel Therese Klemetsrud Helene Jonassen Marianne Hiorth Anna-Lena Kjøniksen Gro Smistad.
The increase in the size of the liposomes after coating with pectin is displayed in Fig. 1b. The liposomes coated with HM-pectin increased .Advantages of pectin coating of liposomes